Influence of Euonymus alatus Sied extracts on MDCK proliferation and high concentration of glucose induced cell injury

Objective. To study the effects of extracts (SML-1, SML-2, SML-3 and SML-4) from Euonymus alatus Sied on cell proliferation and on renal tubular cell (Madin-Darby Canine Kidney, MDCK) injury induced by high concentration of glucose. Methods. MTT assay was used to evaluate the effects of herbal extracts on cell proliferation and viability in MDCK cells after exposure to high concentration of glucose. Lactate dehydrogenase (LDH), N-acetyl-β-amino glucokenase (NAG) and alkaline phosphatase (ALP) in the culture medium were determined by enzyme-linked kits assay. Results. MTT results showed the cell inhibitory rates in drug groups were significantly lower than that of the positive control (cisplain) in normal MDCK cells except SML-1 (100 μg/ml) and SML-2 (100 μg/ml). Following high concentration of glucose exposure there was a significant rise in LDH release rate and NAG concentration, and a drop in ALP concentration. The extracts were proved to be effective in reducing these glucose-induced injurys in MDCK cells. Conclusion. Along with high concentration of glucose, extracts from Euonymus alatus Sied may be useful therapeutic agents in treating renal tubular cell injury. Our findings also support that MDCK were sensitive to high concentration of glucose and the changes of cell permeability and antioxidant capacity induced by high concentration of glucose might be linked to the pathogenesis of diabetic nephropathy. [Life Science Journal. 2008; 5(4): 41 – 46] (ISSN: 1097 – 8135).